P-200 Tailoring IVF laboratory key performance indicators of the Vienna consensus in testicular sperm aspiration cases

Abstract Study question Are the IVF laboratory KPIs of Vienna consensus equally applicable to ICSI cycles performed with ejaculated or testicular sperm aspiration (TESA) samples? Summary answer In addition standard cycles, are largely TESA cases, except for 2PN fertilization and day 3 development rates. What is known already The ESHRE SIG Embryology Alpha Scientists in Reproductive Medicine produced first international on a systematic set KPIs. These efficiency measures can reveal possible impact extrinsic factors, such as operator skills culture media, process. However, control intrinsic factors – i.e., specific gamete characteristics Consensus focused “reference population” defined by female age ≤39years, exclusion PGT cases use own fresh oocytes sperm. This leaves relevance other patient populations, including uncertain. design, size, duration was retrospective, single-center cohort analysis 1678 ART carried out between 2010 2022. Treatments involving were 105 1573, respectively. Participants/materials, setting, methods Inclusion criteria indication IVF/ICSI spermatozoa blastocyst all embryos formed each cohort. Oocyte donation not included. Ovarian stimulation either recombinant-FSH hMG, combined GnRH prevent spontaneous LH surge. Fertilization collected achieved ICSI. Main results role chance Both maternal paternal comparable two groups. rate lower (N = 593/915, 64.8% versus N 7276/10350, 70.3%; p < 0.01), while 1PN higher 32/915, 3.5% 246/10350, 2.4%; 0.04). Other developmental rates comparable: 3PN 24/915, 2.6% 341/10350, 3.3%); microinjection damage 52/915, 5.7% 632/10350, 6.1%); failed 4/105, 3.8% 61/1573, 3.9%); cleavage 579/593, 97.6% 7105/7276, 97.6%); 2 318/593, 53.6% 3887/7276, 53.4%); 248/593, 41.8% 3279/7276, 45.1%); total good 147/361, 40.7% 1728/3944, 43.8%). Compared Consensus, outcomes within normal ranges competency benchmark values, slightly below thresholds. Limitations, reasons caution study, especially because retrospective small sample requires external independent validation Wider implications findings study confirms robustness recommendations monitoring performance, highlighting need fine-tuning individual indicators populations. Trial registration number